Fig. 1: 2CLCs and totipotent embryos display slow replication fork speed.
From: DNA replication fork speed underlies cell fate changes and promotes reprogramming
a, Experimental setup for isolation of ESCs (GFP−) and 2CLCs (GFP+) based on FACS. b–g, DNA fiber analysis of pluripotent and totipotent cells by sequential labeling of nascent DNA. Representative fiber images (b) and quantification results of fork speed (c) from ESCs and 2CLCs are shown. d, Distribution of patterns of replication derived from fiber analyses from ESCs and 2CLCs. e,f, Representative fiber images (e) and quantification results of fork speed (f) from mouse embryos at the indicated developmental stages. g, Quantification of the IOD at the indicated stages of mouse preimplantation embryos. In c and f, the red line indicates the median. In g, the boxplots show the median and the IQR and whiskers depict the smallest and largest values within 1.5 × IQR. In c, f and g, statistical analyses were performed with a two-sided Wilcoxon’s rank-sum test. In d, statistical analyses were performed using a two-sided binomial test. In b and e, scale bars, 5 μm.
