Fig. 5: Improvement of the developmental potential of SCNT-derived embryos.

a, SCNT embryos from cumulus cells treated with HU for 24 h after NT. Reprogramming efficiency was estimated by calculating the developmental rate of NT-derived embryos to the blastocyst stage. Barplots indicate the percentage of developmental efficiency of ten (control) and seven (10 μM HU) independent experiments. Each dot indicates the percentage obtained in each of these experiments and n indicates the total number of activated oocytes analyzed. Statistical analyses were performed using the z-score test for two population proportions (two tailed). b, Principal component (PC) analysis depicting the transcriptional profile of all NT embryos 28 h after activation, analyzed by single embryo RNA-seq, in comparison with wild-type embryos³⁸. Note that NT-derived embryos cluster at the corresponding developmental time at which they were collected, indicating transcriptional reprogramming. c, Boxplot depicting the expression levels across RRRs in cumulus cells and control and HU-treated NT embryos. Each dot represents individual embryos (biological replicates). The boxplots indicate the first and third quartiles as the lower and upper hinges and the whiskers extend to the lowest and highest value no further than 1.5 × IQR.