Extended Data Fig. 4: Accumulation of ID4-eGFP positive germ cells in Dnmt3A^KO testes.

a, Representative image of SOX9 (Sertoli cell marker) staining on testis sections from Dnmt3A^KO, Prdm1-Dnmt3A^cKO and controls at 10 dpp, 25 dpp, 6 weeks, 8–9 weeks and 6 months. Scale, 20 μm. b, (Left) Representative microscopy images of double-immunofluorescence detection of SOX9 (Sertoli cell marker) and ID4-eGFP (SSC) at 10 dpp. Scale, 20 μm. (Right) Quantification of the number of ID4-eGFP positive cells per mm² of tubule in Dnmt3A^KO and WT. Data are mean ± SD (black bar). WT, n = 3 and Dnmt3A^KO, n = 3 (one-sided student t-test over WT). c, (Left) Representative FACS analysis plots of live ID4-eGFP-positive gated testicular cells from 10 dpp mice. ID4-eGFP cells were divided into three classes: GFP dim, GFP med and GFP bright. (Right) Representative count of cell numbers in each GFP subclass in Dnmt3A^KO and WT. (Far right) Cell count in percentage for each GFP subclass in WT and Dnmt3A^KO for two biological replicates. #1 et #2 represent individual replicates.