Extended Data Fig. 4: Comparison of growth advantage in CCL22-Pro79arg, wild type, and empty vector transduced cells. | Nature Genetics

Extended Data Fig. 4: Comparison of growth advantage in CCL22-Pro79arg, wild type, and empty vector transduced cells.

From: CCL22 mutations drive natural killer cell lymphoproliferative disease by deregulating microenvironmental crosstalk

Extended Data Fig. 4

Cytokine independent assay in CCR4 expressing (a) Ba/F3 cells with murine Il-3 or recombinant CCL22 proteins and (b) NK-92 cells with human IL-2, co-expressing wild type or mutant CCL22 (Pro79Arg, Leu45Arg), or GFP-expressing lentiviral vector (Empty vector). No cytokine independent growth was observed. The mean (± SD) cell count (x 1,000,000) is shown (n = 3). c,d, No growth advantage was observed by adding hIL-15 and/or hIL-18 to engineered NK-92 cells. The mean (± SD) cell count (x 1,000,000) is shown (n = 3). e, Recombinant murine IL-15 showed cross-reactivity to human engineered NK-92 cells with lower efficiency than human IL-15. The mean (± SD) relative proliferation rate of murine IL-15 compared to human IL-15 is shown (n = 6, except for empty vector at day6 n = 4 due to technical issue).

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