Extended Data Fig. 7: PCR validation of transgenic insertions. | Nature Genetics

Extended Data Fig. 7: PCR validation of transgenic insertions.

From: SunUp and Sunset genomes revealed impact of particle bombardment mediated transformation and domestication history in papaya

Extended Data Fig. 7: PCR validation of transgenic insertions.

Three transgenic insertions, 9789 bp functional insertion including CP, 1533 bp tetA insertion and 290 bp nptII insertion were amplified in Sunset and SunUp. Three insertions can be amplified in SunUp but not in Sunset by using the 5 primer pairs listed in Supplementary table 66. The experiment was repeated three times and the same results were obtained. PCR products were confirmed by Sanger-sequencing, and the result was used to correct the assembly sequence.

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