Extended Data Fig. 2: PCR validation of transgenic insertion sites in Sunset. | Nature Genetics

Extended Data Fig. 2: PCR validation of transgenic insertion sites in Sunset.

From: SunUp and Sunset genomes revealed impact of particle bombardment mediated transformation and domestication history in papaya

Extended Data Fig. 2

a, The schematic diagram for the 1.64 Mb insertion in SunUp. Primers were designed to amplify the insertion loci P3, and sequences of both sides were defined as P1 and P2 in SunUp and Sunset. b, The insertion loci P3 can be amplified in SunUp but not in Sunset. PCR products for band P3 were confirmed by sanger-sequencing, and the result was consistent with assembly sequence. The experiment was repeated three times and the same results were gotten. c-d, The dot plot for both border sequences in SunUp and Sunset which were marked as Sunset P1/P2 and SunUp P1/P2.

Back to article page