Extended Data Fig. 5: NFIA and NFIX support an adult-type erythroblast transcription program.
From: Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells
a, Clustering analysis of 430 NFIA and NFIX co-depletion affected genes in HUDEP2 cells. A total of four distinct expression patterns are identified. Boxplot showing expression levels of genes in Group 1 (n = 234) and Group 2 (n = 174) display a correlation with the dosage of total NFI proteins. Y-axes represents z-scores of gene abundance. Lower whisker, smallest observation greater than or equal to lower hinge - 1.5 x Interquartile range (IQR); lower hinge, 25% quantile; median, 50% quantile; upper hinge, 75% quantile; upper whisker, largest observation less than or equal to upper hinge + 1.5 x IQR. b–d, Scatter plot showing the comparison of adult globin genes (HBA1/2, HBB, and HBD) and red cell differentiation-related genes (GATA1, ALAS2, and BAND3) in representative control, NFIA and NFIX single or co-depleted (sgNFIA&NFIX) HUDEP2 cells.
