Extended Data Fig. 1: Chromosome specific oligo probes to S. spontaneum accessions with x = 8. | Nature Genetics

Extended Data Fig. 1: Chromosome specific oligo probes to S. spontaneum accessions with x = 8.

From: Genomic insights into the recent chromosome reduction of autopolyploid sugarcane Saccharum spontaneum

Extended Data Fig. 1: Chromosome specific oligo probes to S. spontaneum accessions with x = 8.

(a) Probes to S. spontaneum SES208 (2n = 8x = 64, x = 8). In I, II, III, IV, and V, S. spontaneum Np-X chromosome-specific oligo probes NpC1, NpC3, NpC5, NpC7, and NpC9 are visualized in red, respectively. S. spontaneum Np-X chromosome-specific oligo probes NpC2, NpC4, NpC6, NpC8, and NpC10 are visualized in gree. S. spontaneum Np-X chromosome-specific oligo probes NpC7 and NpC8 are visualized in cyan in I, III, and V. Karyotypes of SES208 by Sschr 01 to Sschr 08 in VI. These results confirm the major chromosomal rearrangement events between Np-X and AP85-441 genomes. Bars = 10 μm. (b)Probes to S. spontaneum 82-114 (2n = 10x = 80, x = 8). In I, II, III, IV, V, VI, and VII, S. spontaneum Np-X chromosome-specific oligo probes NpC1, NpC3, NpC5, NpC7, NpC9, NpC5 and NpC8 are visualized in red, respectively. S. spontaneum Np-X chromosome-specific oligo probes NpC2, NpC4, NpC6, NpC8 and NpC10 are visualized in green. S. spontaneum Np-X chromosome-specific oligo probes NpC7 and NpC9 are visualized in cyan in VI and VII. Karyotypes of S. spontaneum 82-114 by Sschr01 to Sschr08 in VIII. The experiments were repeated independently at least three times with similar results. These results confirm the major chromosomal rearrangement events between Np-X and AP85-441 genomes, Bars = 10 μm.

Back to article page