Fig. 1: Cell-type specificity of SARS-CoV-2 regulators identified by CRISPR screens. | Nature Genetics

Fig. 1: Cell-type specificity of SARS-CoV-2 regulators identified by CRISPR screens.

From: Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs

Fig. 1

a, Schematic of pooled screen pipeline to identify SARS-CoV-2 regulators in Vero E6 cells. b, Scatter plot showing the gene-level mean z-scores of genes when knocked out in Vero E6 cells. The top genes conferring resistance to SARS-CoV-2 are annotated and shown in blue (n = 20,928). c, Comparison between this Vero E6 screen to the Vero E6 screen conducted by the Wilen lab20. Genes that scored among the top 20 resistance hits and sensitization hits in both screens are labeled. Pearson’s correlation coefficient r is indicated (n = 20,928). d, Venn diagram comparing hits across screens conducted in Vero E6, A549 and Huh7.5 (or Huh7.5.1) cells (ectopically expressing ACE2 and TMPRSS2 or not)16,17,18,19,20,21. The top 20 genes from each cell line are included, with genes considered a hit in another cell line if the average z-score was >3.

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