Extended Data Fig. 9: Characterization of proviral genes identified by CRISPRa.
a-b. ACE2 expression in CRISPRa cell lines. Calu-3-Cas9 cells were stably transduced to express 2 different sgRNAs (g1, g2) per indicated gene and selected (parallel samples from Fig. 8). a. The cells were lysed and expression levels of ACE2 were analyzed, Actin served as a loading control A representative immunoblot (out of 2 independent experiments) is shown. b. ACE2 surface staining using Spike-RBD-mFc. Mean and SEM of 3 independent experiments. Statistical significance was determined by a two-sided t-test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). c. Dot plot depicting the expression levels of the best validated proviral genes in the different cell types from the respiratory epithelium, from Chua et al. data set33. Expression levels in COVID-19 versus healthy patients are color coded; the percentage of cells expressing the respective gene is size coded, as indicated.
