Fig. 6: NMD targets OAS1 isoforms with short exon 3 upregulated by the risk rs1131454-A allele. | Nature Genetics

Fig. 6: NMD targets OAS1 isoforms with short exon 3 upregulated by the risk rs1131454-A allele.

From: Genetic regulation of OAS1 nonsense-mediated decay underlies association with COVID-19 hospitalization in patients of European and African ancestries

Fig. 6: NMD targets OAS1 isoforms with short exon 3 upregulated by the risk rs1131454 -A allele.The alternative text for this image may have been generated using AI.

a, RNA-seq plots showing OAS1 expression and splicing patterns in HeLa cells (rs10774671-AA, OAS1-p42) targeted by siRNA KD of NMD-pathway genes SMG6 and SMG7 or by scrambled siRNA (Neg Ctrl). b, Expression of both long and short OAS1-p42 isoforms is increased in HeLa cells with siRNA KD of NMD genes. c, Schematics for characterizing effects of KD of NMD genes on the expression of long and short exon 3 in the context of OAS1-p42 (in A549) and OAS1-p46 (in HT1376). d,e, Downregulation of NMD-pathway genes (SMG6, SMG7 and UPF1) targeted by siRNA KD in A549 cells. Expression of both long and short exon 3 OAS1-p42 isoforms is increased in cells with siRNA KD of NMD genes. Tri-KD, triple KD. f,g, Downregulation of NMD-pathway genes (SMG6, SMG7 and UPF1) targeted by siRNA-KD in HT1376 cells. Only expression of short-exon 3 OAS1-p46 isoform is increased by siRNA-KD of NMD genes. Expression in three biological replicates was analyzed by qRT-PCR and normalized to an endogenous control (HPRT1). The dot plots are presented with means and s.d.; P values are for unpaired, two-sided Student’s t tests.

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