Extended Data Fig. 7: Loop anchors with strong single-oriented CTCF binding can constitute the source of weaker loops in the nonconvergent direction.

a-c. Three different examples of developmental gene loci with loop anchors that display unidirectional CBS and are engaged in both convergent and nonconvergent loops. Hi-C interaction matrices and CTCF ChIP-seqs are from the mESC dataset in²⁶. CBS orientations are displayed in red and blue for positive and negative strands respectively and were calculated using FIMO⁵² (see methods). Dark red and dark blue arrowheads indicate convergent and associated nonconvergent loops respectively. Insulation scores, boundaries and boundary scores were calculated with FAN-C⁴⁹ (see methods). Black boundary bars depict boundaries that do not contain divergent CBS pairs in their vicinity.