Extended Data Fig. 1: Structural and molecular comparison between Delbs and DelB mutants. | Nature Genetics

Extended Data Fig. 1: Structural and molecular comparison between Delbs and DelB mutants.

From: In vivo dissection of a clustered-CTCF domain boundary reveals developmental principles of regulatory insulation

Extended Data Fig. 1: Structural and molecular comparison between Delbs and DelB mutants.

a. Schematic shows Epha4 enhancers (in dark gray), Epha4 gene (in pink), Pax3 gene (in purple), EP boundary (in black) and genomic rearrangements in the DelBs and Delb mutants (light gray rectangles). b-d. Capture Hi-C (cHi-C) maps in E11.5 limbs in WT (B) DelBs (C) and DelB (D) mutants (data from28). Genomic coordinates in C and D correspond to custom DelBs and DelB genomes for the captured region, respectively. Pax3 WISH (right panel). Note how the presence of the EP boundary is sufficient to block the functional interaction between the Epha4 enhancers and the Pax3 gene, thus preventing its misexpression. Cen, centromeric. Tel, telomeric. d-Limbs, distal limbs. Scale bars, 250 μm.

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