Fig. 2: Maintenance of epigenetic memory at ICRs is sequence dependent in mESCs. | Nature Genetics

Fig. 2: Maintenance of epigenetic memory at ICRs is sequence dependent in mESCs.

From: DNA sequence and chromatin modifiers cooperate to confer epigenetic bistability at imprinting control regions

Fig. 2

a, Tabular summary of methylation analysis for all Airn-ICR fragments schematically indicated on the left. In addition, fragment length, CpG densities and GC content is shown for each fragment. Same representation as in Fig. 1b. b, Methylation analysis for the shuffled Airn ICR. Same representation as in Fig. 1c. CpG positions marked with ‘x’ correspond to unaligned nucleotides due to sequencing errors. c, ChIP-qPCR measurements at the shuffled Airn ICR at the ectopic site compared to the endogenous Airn ICR. Data points show individual technical replicates. H3K9me3, blue; H3K4me2, orange. d, Methylation analysis for the shuffled Airn ICR with reconstituted ZFP57 binding sites. Same representation as in panel b. e, Methylation analysis for the shuffled Airn ICR integrated to murine erythroleukemia (MEL) cells shows maintenance of DNA methylation.

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