Fig. 4: CRISPR screens identify regulators of epigenetic memory at ICRs in mESCs. | Nature Genetics

Fig. 4: CRISPR screens identify regulators of epigenetic memory at ICRs in mESCs.

From: DNA sequence and chromatin modifiers cooperate to confer epigenetic bistability at imprinting control regions

Fig. 4

a, Experimental overview of targeted CRISPR screens using multiple premethylated ICR reporters. Gating strategy is described in Extended Data Fig. 7a and Methods. b, Overview hits from CRISPR screens in three ICR reporter cell lines grown in serum conditions. Blue dots indicate genes with a P value < 0.01 calculated using MAGeCK RRA (robust rank aggregation). Dashed lines indicate the P value threshold at 0.05. c, Heatmap showing potential candidates from all CRISPR screens. Color indicates the summarized log fold change across all guides for a given gene, as determined by MAGeCK. Enrichments were calculated combining all replicates for one comparison, using the GFP-enriched fraction against the unsorted pool of cells. Asterisk indicates P < 0.05 using MAGeCK robust rank aggregation. See corresponding panel b and Extended Data Fig. 8b,d. Exact P values can be found in Supplementary Table 1.

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