Extended Data Fig. 2: Stable methylation maintenance at ectopic ICRs. | Nature Genetics

Extended Data Fig. 2: Stable methylation maintenance at ectopic ICRs.

From: DNA sequence and chromatin modifiers cooperate to confer epigenetic bistability at imprinting control regions

Extended Data Fig. 2

a) Summary table for the methylation analysis of the Airn ICR after long term culture (over 20 consecutive passages), random integration to a different genomic site, during differentiation to neuronal progenitor cells (NPCs), or after culturing in 2i for 10 days. b-d) Single molecule measurements from bisulfite PCR corresponding to data summarized in a. e) Methylation analysis for the ectopic and endogenous Airn ICR after CRISPR-mediated Zfp57 KO. Triangles indicate CpGs within ZFP57 motifs. The region analysed by bsPCR is indicated. f) Schematic overview of Airn ICR fragments tested in the study (top) and single-molecule bisulphite PCR results from the individual fragments (below) g) Summary table for the methylation analysis of the H19 ICR fragments, including size, CpG density and GC content information. h) Single molecule measurements from bisulfite PCR corresponding to data summarized in g.

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