Extended Data Fig. 10: Characters of TaSD6.
From: Antagonistic control of seed dormancy in rice by two bHLH transcription factors
a, Phylogenetic analysis of SD6 in different cereal crops by the Neighbor-Joining method. Branch lengths represented extents of homology between branches. b, Characterization of SD6 homologs in wheat. Exons of TaSD6 are shown as blue squares, and the target site of CRISPR/Cas9 is marked with red lines in the conserved region encoding the helix-loop-helix DNA-binding domain. c, PCR/RE analysis of seven mutants at the target site of TaSD6. PCR was performed using A, B, and D sub-genome-specific primers and the products were digested with restriction enzyme Alw44I. d, Detailed sequence of the triple-recessive bi-allelic mutant (T0-1) at the target site. The red lowercase letters and short lines represent 1-bp insertions and deletions, respectively. e, Seed germination rates of wild type and the triple-recessive homozygous mutant (tasd6) at each day after imbibition in 2021. Germination test in e was performed with eight biological replicates. Data are presented as mean values ± s.d. f, Seed germination status of wild type and the triple-recessive homozygous mutant (tasd6) after 4-day imbibition. g, Seed germination rates of dormancy-released wild type and tasd6 via after-ripening at each day after imbibition in 2021. Germination test in g was performed with four biological replicates. h, Spike spouting rate of wild type and tasd6 after 6-day imbibition in 2021. Each spike was harvested and incubated under high humidity. i, Grain number per spike of wild type and tasd6. Analysis in h and i was repeated with at least twenty-five biological replicates. Scale bar 1 cm. Box plots denote median (horizontal line) with minima to maxima whiskers. All P values are based on two-tailed Student’s t-tests.
