Extended Data Fig. 1: scMPRA measures cell-type specific CRS activity.
From: A single-cell massively parallel reporter assay detects cell-type-specific gene regulation
(a) UMAP of the single-cell transcriptome from the mixed-cell experiment. 105 out of 3417 cells (3%) are labeled by both K562 and HEK293 cell genes. (b) UMAP of the mixed-cell experiment with cells marked by other representative markers for K562 and HEK293 cell expression. (c, d) Histogram of the number of plasmids (unique cBC-rBC pairs) transfected into K562 cells and HEK293 cells. (e, f) Histogram of the mean number of rBC per cBC (CRS) per cell for K562 cells and HEK293 cells. (g, h) Correlation of bulk MPRA versus scMPRA where only the scMPRA data has been UMI normalized (i, j) Scatterplot of scMPRA reproducibility for housekeeping and developmental promoters in K562 cells and HEK293 cells.
