Extended Data Fig. 7: DNE can activate Sox2 across CTCF-mediated insulation. | Nature Genetics

Extended Data Fig. 7: DNE can activate Sox2 across CTCF-mediated insulation.

From: Enhancer–promoter interactions can bypass CTCF-mediated boundaries and contribute to phenotypic robustness

Extended Data Fig. 7: DNE can activate Sox2 across CTCF-mediated insulation.

a Virtual 4C plot using Sox2 and DNE viewpoints with signal plotted at each DpnII fragment. This same signal at 5 kb overlapping bins is shown in the main figure. This representation highlights how, despite massively reduced, Sox2 can interact with DNE in all lines assessed. Signal is shown as average of the 2 replicates of each genotype. Region surrounding viewpoint was removed from analysis. Dotted lines highlight DNE in the Sox2 viewpoint (left), and Sox2 in the DNE viewpoint (right). Virtual 4C signal is shown as average of the 2 replicates of each genotype at DpnII fragment resolution. b IF of E11.5 embryos stained with an antibody targeting SOX2. Scale bar represents 500 µm. 2 embryos of each genotype were stained and imaged.

Back to article page