Extended Data Fig. 7: BRCA1+/mut vascular cells express elevated levels of NGF which increases branching morphogenesis.
From: Preneoplastic stromal cells promote BRCA1-mediated breast tumorigenesis
a) UMAP projection of vascular cell states identifying 3 endothelial cell states, 2 pericyte cell states, and lymphatic cells. b) Violin plots of marker genes with enhanced expression in each vascular cell state cluster. c) Volcano plot showing differentially expressed genes between NonCarrier and BRCA1+/mut pericytes, the Wilcoxon rank sum test is used to determine differentially expressed genes, adjusted p values are determined using the Bonferroni method for multiple testing correction. d) Schematic for the generation of hydrogel branching assays. e) Representative images of a BRCA1+/mut organoid in hydrogel branching assay at days 6-9 after seeding. Scale bars = 200 μm. f) Branch growth curves of n = 6 control and n = 6 NGF (100 ng/ml) treated hydrogel branching assay. P value was calculated using CGGC permutation (two-sided) test43.
