Extended Data Fig. 7: Lineage-selective roles of MLL3/4 enzymatic activities in early embryonic development.
a, Schematic representation of Mll3 flox and deletion, as well as Mll4SET flox, deletion and KI alleles10. Locations of PCR genotyping primers are indicated by arrows. The MF primer is specific to the KI allele. b, PCR genotyping of E9.5 yolk sac-removed embryos using primers showed in Extended Data Fig. 7a as well as primers detecting Sox2-Cre allele. Sizes of PCR products are indicated on the left. c, Whole cell lysates prepared from E9.5 yolk sac-removed embryos were analyzed with immunoblotting using indicated antibodies. d, Sox2-Ctr and Sox2-dKI embryos were collected at E9.5 for RNA-seq. Levels of expressed genes were presented as scatter plots. Pearson correlation coefficient (R) and the corresponding two-sided P value are shown. The number of genes in each group is indicated in parentheses. e, GSEA of 11,467 expressed genes in E9.5 embryos on developmental terms. Nom p-value was determined by a 1000-fold permutation test; FDR q-value was adjusted for multiple hypotheses testing. NES, normalized enrichment score. Statistically significant data are highlighted in bold. f, Heat maps of gene expression level in E9.5 embryos for markers of germ layer derivatives. Data from 3 embryos were presented for each genotype.
