Extended Data Fig. 5: GJA1 expression in peri-capsular cells expressing VSNL1 (ZG marker) co-localizes with wheat germ agglutinin (WGA; cell membrane marker). | Nature Genetics

Extended Data Fig. 5: GJA1 expression in peri-capsular cells expressing VSNL1 (ZG marker) co-localizes with wheat germ agglutinin (WGA; cell membrane marker).

From: Somatic mutations of CADM1 in aldosterone-producing adenomas and gap junction-dependent regulation of aldosterone production

Extended Data Fig. 5

IFC staining of CYP17A1, VSNL1 (a ZG marker), and GJA1 was performed on serial sections from an adrenal harboring an APA. IFC staining color-coded as indicated in image. A no primary (1°) antibody control was performed to take into account autofluorescence that occurs in the region. GJA1 punctate expression, annular GJ (white arrow), and GJ plaque (yellow arrow) co-localizes with WGA in VSNL1 positive (+ve) cells. Left, scans of the serial sections. Right, zoomed image within boxed regions in scans. Bottom, image of the CYP17A1 positive cells shown in Extended Data Fig. 4.

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