Extended Data Fig. 6: Presence of annular GJA1 in APM regions (CYP11B2-expressing cells).

IFC staining of CYP11B2 and GJA1 was performed on serial sections of the same adrenal shown in Extended Data Fig. 5. IFC staining is color-coded as indicated in image. GJA1 expression in APM regions was lower compared to adjacent adrenal cortex. Annular GJs (red arrows) were present in APM. Top, scan of the serial adrenal section; Bottom, zoomed images of ZF region and APM regions highlighted in scan.