Extended Data Fig. 6: Virtual 4C contacts generated from Capture Hi-C datasets produced out of control stembryos at 48 h, 96 h and 144 h.
From: Sequential and directional insulation by conserved CTCF sites underlies the Hox timer in stembryos
The contacts between the various viewpoints (Hoxd genes shown on top) and the Evx2-Hoxd12 region showed a progressive decrease upon activation of the cluster, particularly visible for both Hoxd8 and Hoxd9. Contacts values were normalized to 48 h for each viewpoint. Asterisks represent significant changes against as determined by the Kruskal-Wallis followed by Dunn’s multiple comparisons test (* is p-value < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001). Box plots with median value and 25–75% percentiles, whiskers represent minimum and maximum (sum of 3 independent replicates).
