Fig. 2: Dynamics and directional cohesin loading at the HoxD cluster.
From: Sequential and directional insulation by conserved CTCF sites underlies the Hox timer in stembryos
a, CTCF ChIP–seq profile using stembryos at 72 h (upper panel). The orientations of CBS1-9 motifs are shown with red and blue arrowheads. Below are cumulative H3K27ac ChIP–seq signals from 72 h to 168 h showing the various points of transitions in transcriptional activation over time. The red domain delineates the initial and rapid phase of acetylation, while the following and more progressive activation phase occurs in the blue domain. Black arrows point to regions where the progression in H3K27 acetylation was delayed (Supplementary Movie 1). b, Timecourse of RAD21 ChIP–seq profiles. CBS number and orientations are as in a. The accumulation of RAD21 outside CBSs is exemplified by the dashed box (quantifications in Extended Data Fig. 2a). c, Timecourse of NIPBL ChIP–seq profiles over Hoxd genes. NIPBL binding corresponds to acetylated regions. Progressive NIPBL enrichment correlates with the activation dynamics of the cluster. Red arrows in a–c highlight two acetylated regions where NIPBL is detected and RAD21 accumulates. Genomic coordinates—chr2:74667374–74767842.
