Extended Data Fig. 6: RAD21 depletion reprograms genome-wide replication timing.
From: Cohesin maintains replication timing to suppress DNA damage on cancer genes
a FACS sorting strategy for replication timing analysis. The black and green circles gate the mClover negative and positive cells, respectively. The red rectangles indicate the six fractions of sorted cells according to DNA content. b A representative profile of replication timing in WT and RAD21-mAC cells (clone 4#) with IAA treatment for 6 hours or 24 hours. c Scatter plot of S50 values between WT K562 replicates (clone 4#) with IAA treatment for 24 hours. The grey region between dashed lines indicates the 95% quantile interval of WT replicates. Legends are described in Fig. 5b and the computation details are described in Methods. d Scatter plot of S50 values between WT and RAD21-mAC cells (clone 4#) with IAA treatment for 6 hours. Legends are described in Fig. 5b. e Distribution of S50 values in IAA-treated WT and RAD21-mAC cells. The p-value from the two-sided Mann–Whitney test is labeled on the top of the plots. f Scatter plot of S50 values between WT mES replicates with IAA treatment for 3 hours. Legends are described in Fig. 5b.
