Extended Data Fig. 1: Cohesin mutations in cancers and construction of RAD21 degron system in K562 cells.
From: Cohesin maintains replication timing to suppress DNA damage on cancer genes
a Mutation frequency of cohesin subunits in dozens of cancer types. Cancer types in the TCGA database with more than 2000 cases and over 0.1% RAD21 mutation are displayed and ranked according to the mutation frequency of RAD21. b Proportions of four types of mutations in cohesin subunits. Mutations are obtained from the Catalogue of Somatic Mutations In Cancer (COSMIC) database. The SIFT tool is used to predict the functional consequence of cohesin mutations, including amino acid insertions, deletions, and substitutions, which identifies deleterious and neutral mutations (See Method for details). The case number of mutations in each subunit is labeled above each pie chart. Subs., substitutions; Indels, insertions, and deletions. c Knock-in and PCR validation strategy for RAD21 tagged with mAID-mClover in K562 cells. The representative PCR plot is shown at the bottom, the result was confirmed by two independent replicates. d Flow cytometry analyzing mClover fluorescence of two RAD21-mAC K562 clones after IAA treatment for the indicated time. e Quantification of γH2A.X foci in WT and RAD21-mAC cells without IAA treatment. The red lines indicate the median number of γH2A.X foci and the examined cell numbers are labeled at the bottom. Two-sided Mann–Whitney test; n.s., no significance.
