Fig. 1: Symmetric segregation of parental histones is required for the balanced inheritance of histone PTMs to daughter cells. | Nature Genetics

Fig. 1: Symmetric segregation of parental histones is required for the balanced inheritance of histone PTMs to daughter cells.

From: Symmetric inheritance of parental histones governs epigenome maintenance and embryonic stem cell identity

Fig. 1

a, Illustration of asymmetric segregation of parental histones H3–H4 to leading strand in MCM2-2A ESCs14 and how this could challenge histone PTM inheritance and daughter cell function. b, Design of SCAR-seq pulse-chase experiments. c–e, Average SCAR-seq profiles of H3K27me3 (c), H3K4me3 (d) and H3K27ac (e) partition in 1-kb windows around replication initiation zones. Partition is calculated as the proportion of forward (F) and reverse (R) read counts ((F − R)/(F + R))14. n = number of initiation zones. Replication fork directionality in WT cells14 measured by Okazaki fragment sequencing (OK-seq)85 is shown for comparison. The average of two biological replicates is shown (see Extended Data Figs. 2 and 3 for individual replicates in two MCM2-2A clones).

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