Extended Data Fig. 8: Sequence and conformation of the D458 ecDNA.
From: Circular extrachromosomal DNA promotes tumor heterogeneity in high-risk medulloblastoma
(a) Reconstruction of the D458 ecDNA from OGM and WGS data. All junctions are supported by WGS discordant and optical genome mapping reads. (b) Chromatin interaction heatmap between co-amplified segments of chr8 and chr14 on the D458 ecDNA. Notable ectopic interchromosomal interactions are indicated here and in Fig. 4f. f: functional, as determined by a significant and D458-specific effect on cell proliferation upon CRISPRi inhibition (Fig. 4g); nf: not identified as functional in the same pooled CRISPRi screen.
