Extended Data Fig. 3: ZNF808 KO and protocol of differentiation toward beta cells.
From: Primate-specific ZNF808 is essential for pancreatic development in humans
a. Differentiation protocol to generate pancreatic endoderm, progenitors and islets from human embryonic stem cells. b. Flow cytometry analysis for the definitive endoderm marker CXCR4. n = 3 independent differentiation experiments, data are presented as mean values ± SEM. Axis labels state the marker and fluorochrome used. (a.u.=arbitrary unit, S1=definitive endoderm stage). c. Flow cytometry analysis for the pancreatic progenitors markers PDX1 and NKX6-1 at S4 (pancreatic progenitors stage). n = 3 independent differentiation experiments, data are presented as mean values ± SEM. Axis labels state the marker and fluorochrome used. (a.u.=arbitrary unit). d. Immunohistochemistry analysis of S3 (posterior foregut stage) monolayer cells for PDX1 and alpha-fetoprotein (AFP); and S4 (pancreatic progenitors stage) monolayer cells for PDX1, NKX6-1, SOX9 and NGN3 (representative of 3 independent differentiation experiments, scale bar = 200 um).
