Extended Data Fig. 3: Validation of CX-5461 signatures in alternative cellular models.
From: The chemotherapeutic drug CX-5461 is a potent mutagen in cultured human cells
a. Aggregated whole-genome mutational profiles of CX-5461-treated HAP1 subclones (n = 2). b. Mutation frequencies normalized by the total duplex bases per sample across different compounds in HAP1 (left) and human induced pluripotent stem cells (hiPSC) (right) (n = 1 per treatment arm). Mutation frequency fold-increases were calculated against respective untreated control (bar top). BaP, benzo(a)pyrene; ETO, etoposide; PDS, pyridostatin. c. Trinucleotide spectrum plots for treated bulk cells by duplex sequencing. d. Unsupervised hierarchical clustering of mutational spectra (six mutation types) collapsed from c. using (1-cosine similarity) as distance matrix. BaP, benzo(a)pyrene; Cis, cisplatin; ETO, etoposide; PDS, pyridostatin.
