Fig. 1: Map-based cloning of the quantitative GLS-resistance gene at the qRgls1 locus.
From: The ZmWAKL–ZmWIK–ZmBLK1–ZmRBOH4 module provides quantitative resistance to gray leaf spot in maize
a, Recombinant-derived homozygous lines with/without Y32 segments and their GLS-resistance performance (n = 1151). The symbols ‘+’/‘−’ indicate the presence/absence of the resistance allele at qRgls1, respectively. b, Schematic diagram of the complementary pCAMBIA3301-ZmWAKLY construct. c–h, Resistance performance of the complementation transgenic lines in self-pollinated (c–e) and T1BC1F1 and T1BC3F1 backcrossed populations (f–h). c,f, Relative expression of ZmWAKL was determined by RT–qPCR (n = 3), and the transgene was confirmed by PCR. d,e, GLS symptoms (d) and scales (e) of B73 and homozygous transgenic lines (n = 297). g,h, DSI values of transgenic and nontransgenic plants in T1BC1F1 (n = 189) and T1BC3F1 (n = 304) segregating populations. i, Schematic diagram of the overexpressed Ubipro:ZmWAKLY-GFP construct. j–o, GLS resistance of the overexpression transgenic plants in self-pollinated (j–l) and backcrossed populations (m–o). j,m, Relative expression of ZmWAKL was determined by RT–qPCR (n = 3), and the transgene was confirmed by immunoblotting with α-GFP antibody. k,l, GLS symptoms (k) and scales (l) of B73 and homozygous transgenic lines (n = 491). n,o, DSI values of transgenic and nontransgenic plants in T1BC1F1 (n = 469) and T1BC3F1 (n = 805) segregating populations. Data are presented as mean values in g and n; means ± s.e. in h and o; means ± s.d. in c, f, j and m. Data in a, e and l are displayed as box and whisker plots with individual data points, the asterisk denotes the mean and the box limits indicate the interquartile range. In c, e, j and l, different lowercase letters indicate a significant difference (P < 0.05) based on one-way ANOVA with Tukey’s test (c,j) or Fisher’s LSD test (e,l). In a, f, g, m and n, statistical significance was determined by a two-sided Student’s t test. Statistical significance was determined by a paired t test in h and o. Scale bars in a, d and k, 15 cm. LB and RB, the left and right T-DNA borders, respectively; NOS, nopaline synthase.
