Fig. 5: Mutational phasing via acute UV damage is established in a single cell cycle.

a, rl20 metric (see Methods) used to determine if there is significant evidence for runs of a single mutation type. The y axis depicts the longest set of runs for a single mutation type, accounting for 20% of all informative mutations. The x axis is the significance (p-value) of seeing such a run length given random assignment to strands, calculated using a two-sided Wald-Wolfowitz runs test. Light blue points represent C > A or G > T mutations (ROS) analyzed for each genome. Red points represent C > T or G > A mutations (UV). b, Example of lesion segregation pattern due to UV induced mutations (top) across all chromosomes of a single mitotic sister, and lack of phasing from ROS induced mutagenesis (bottom) in the same sister. Reference cytosine mutations are shown as yellow dots, whereas reference guanine residues are in blue. The y axis represents log2(distance to nearest neighbor), with G mutation distances converted to negative values to distinguish them from C residues. Chromosomal boundaries are denoted by black vertical dashed lines and chromosomes noted between the tracks. Horizontal dashed line represents a distance of 0. c, Theoretical distribution of mutation phasing in lesion segregation (upper) and no phasing (lower) in 10-Mb genomic tiles. d, Sampling of the distributions of C using the same number of tiles actually profiled in the data (gray), and distribution of the skew from 10-Mb genomic tiles for all 14 genomes profiling acute UV (upper, red) and chronic ROS (lower, light blue) mutagenesis. e, QQ plot comparing distributions of ROS, UV and random sampling of the respective models in teal, red and gray respectively.