Extended Data Fig. 6: Validation of cofactor heterogeneity at early timepoint.
(a) Quantification of change in GFP signal in each of the GAL4-AD lines after CDK8i treatment (24 hrs) or infection with guides targeting CDK8 and CCNC (day 5 after sgRNA infection). Fold change was calculated by comparison to SAFE guide control or DMSO treatment. N = 3 guides or independent treatments. Error bars = S.E.M. Representative FACS plots that were used to calculate the change in GFP signal are shown. (b) Quantification demonstrating concordant change in GFP signal in each of the GAL4-AD lines at 24 hrs after treatment with dTAGV-1 to degrade MED25, and infection with guides targeting MED25 at day 5 after sgRNA infection. Fold change was calculated by comparison to SAFE guide control or DMSO treatment. N = 3 guides or independent treatments. Error bars = S.E.M. Representative FACS plots that were used to calculate the change in GFP signal are shown.
