Extended Data Fig. 2: A Mitosis instead of Meiosis system in inbred tomato.
From: Harnessing clonal gametes in hybrid crops to engineer polyploid genomes
a, Fruit shape overview of wild type, spo11-1-1, rec8-1-1, tam-6, spo11-1 rec8, spo11-1 tam, rec8 tam and spo11-1 rec8 tam mutants. Scale bar = 1 cm. b, Alexander staining results of wild type, single spo11-1-1 mutants, single rec8-1 mutants, single tam-6 mutants, double spo11-1 rec8 mutants and triple spo11-1 rec8 tam mutants’ pollen. Scale bar = 200 µm. c, Single pollen diameter distribution from single flowers of wild type (n = 12191) and triple spo11-1 rec8 tam (inbred MiMe) mutant (n = 12149). d, Seed number per single fruit of wild type (n = 42, 11.74 ± 1.54 SEM), spo11-1 (n = 67, 0 ± 0 SEM), rec8 (n = 89, 0 ± 0 SEM), tam (n = 67, 0.88 ± 0.34 SEM), spo11-1 rec8 (n = 85, 0 ± 0 SEM), spo11-1 tam (n = 41, 0 ± 0 SEM), rec8 tam (n = 43, 0 ± 0 SEM) and spo11-1 rec8 tam (n = 153, 0.84 ± 0.11 SEM). Each dot indicates the seed number of an individual fruit, the solid line represents the median, boxes show quartiles and whiskers show maximum and minimum values. P values are from Ordinary one-way ANOVA followed by Šídák’s multiple comparisons test. ‘ns’ means no significance, and **** indicates a P value < 0.0001. e, Flow cytometry analysis of diploid parent Micro-TomMiMe (black) and tetraploid Micro-TomMiMe offspring (red). Y axis of the histogram represents the events number and X axis of the histogram represents the intensity of DAPI.
