Fig. 1: Comprehensive deletion and transcriptome analysis of ISWI subunits in mESCs.
From: Systematic assessment of ISWI subunits shows that NURF creates local accessibility for CTCF
a, MS quantification of proteins co-immunoprecipitated using an anti-SNF2H antibody in WT and Snf2h∆ cells. Highlighted in red are proteins with P < 0.01 and log2(FC) (WT/Snf2h∆) > 1. Only the names of ISWI subunits are shown. Statistical significance was calculated using a two-sided t-test with Benjamini–Hochberg correction for multiple comparisons (Methods). b, Western blot for ISWI subunits and controls (LAMIN B, CTCF) in all deletion cell lines and WT control. While the deletions show overall no effect on other subunits, we note a change in band stoichiometry for CECR2, ACF1 and BPTF in some of the mutant lines. Blots are representative of at least two experiments. c. Number of DEGs (Methods) upon deletion of ISWI subunits, upregulated in red and downregulated in blue. d, Heatmap of DEGs shown as log2(FC) with respect to parental cell line control. DEGs are clustered based on expression changes, with cluster numbering indicated on the left.
