Fig. 5: BPTF-dependent accessibility impacts nuclear organization, localization of cohesin and the cohesin-release factor WAPL despite persistent CTCF binding.
From: Systematic assessment of ISWI subunits shows that NURF creates local accessibility for CTCF
a, Boxplot (as in Fig. 4a) summarizing changes in CTCF ChIP–seq and ATAC–seq signal for CTCF sites that retain binding upon loss of BPTF, expressed as log2(FC) in respect to WT control cells. Measurements are shown for all sites that retain binding (left) and divided into groups 1–4 based on their accessibility changes upon loss of BPTF. b, Changes in observed/expected interactions at CTCF sites not changing in binding divided by group (as in a) following BPTF depletion, SNF2H depletion and CTCF auxin-mediated degradation (48 h), measured using Hi-C (ratios over respective controls are reported), at 10 kb resolution. Canonical motif orientation (5′–3′) indicated by the arrow. c, Average ChIP–seq signal for RAD21 (top) and WAPL (bottom), at CTCF sites that retain binding, grouped by changes in chromatin accessibility (as in a). Canonical motif orientation (5′–3′) indicated by the arrow.
