Extended Data Fig. 1: In contrast to SNF2H, deletion of SNF2L causes minor effects at the level of gene expression and chromatin accessibility in mES cells.
From: Systematic assessment of ISWI subunits shows that NURF creates local accessibility for CTCF
a. Gene expression (log2 RPKM) distribution in WT mES cells. Expression level of Snf2l and Snf2h are highlighted. b. Western blot detection of SNF2H and SNF2L (upper and lower blot, respectively) protein levels in WT and deletion lines. Blots are representative of two independent experiments. c. RNA changes in SNF2L deletion line are shown as MA plot. Differentially expressed genes (Methods) are reported. d. RNA changes in SNF2L deleted line (y axis) vs SNF2H deleted line (x axis) are shown as density scatter plot. e. ATAC changes in SNF2L deleted line (y axis) vs SNF2H deletion line (x axis) are shown as density scatter plot. f. Average ATAC signal at CTCF-bound sites in WT, Snf2lΔ and Snf2hΔ cells. Canonical motif orientation (5′ to 3′) indicated by the arrow.
