Extended Data Fig. 3: Culture-associated events in mtDNA.
From: Mitochondrial DNA mosaicism in normal human somatic cells
a, Experimental design for assessing culture-associated events5,6. A total of 47 pairs of mother–daughter clones were obtained from ten mother clones. b, Linear correlation between clone-VAFs of mother clones and median clone-VAFs of daughter clones. The gray line represents the diagonal line y = x. Pearson’s correlation coefficient and P value are provided. Two-sided Pearson’s correlation. c, Comparison of clone-VAFs between mother and daughter clones for 49 variants. The mother clone and variants are provided for each panel. Boxplots illustrate median values with IQRs and whiskers (1.5× IQRs). d–g, Comparison between 431 clones from single-crypt-derived organoids (20 individuals) and 432 patches from colon crypts obtained via LCM (42 individuals)72, including linear correlation between age and average SVAF of clones from the individual (d), mutational spectrum (e), the proportion of mutations classified based on functional consequences (f) and clone-VAF distribution (g). d, The gray line and shaded area represent the regression line and its 95% confidence interval. Vertical lines indicate the range of SVAF per clone in an individual.
