Extended Data Fig. 7: Compartmentalization and TAD establishment following YY1 depletion.
From: YY1-controlled regulatory connectivity and transcription are influenced by the cell cycle
a, YY1 ChIP–qPCR of synchronized, sorted prometaphase cells after mitosis-to-G1 depletion of YY1 (n = 3 biological replicates). b, Bar plot of the percent of cells in G1 after 2-hour release from nocodazole arrest after mitosis-to-G1 depletion of YY1, as assessed by DAPI signal from flow cytometry (two-sided Mann–Whitney U test). c, Micro-C contact probability curves for all biological replicates. d, Representative Micro-C contact maps with tracks of compartmentalization, with positive eigenvector 1 values (EV1) corresponding to A compartment and negative values corresponding to B compartment. e, Heatmap of Pearson correlations of subcompartment eigenvector 1 (EV1) values between biological replicates. f, Histogram plot of EV1 values after mitosis-to-G1 depletion. g, Histogram of log2 insulation score values for all boundaries detected in the −auxin_m contact map. h, Representative Micro-C contact map annotated with example TAD calls. Tracks show log2 insulation score values. i, Aggregate domain plot for all TADs called in control contact map, centered on upstream and downstream boundaries. j, Box plot showing loop strengths of all mid G1-detected YY1−YY1 loops weakened by mitosis-to-G1 depletion of YY1 (two-sided Mann–Whitney U test). k, Pileup plots corresponding to loops included in j.
