Extended Data Fig. 10: Next-generation sequencing of base edits across 45 variants modulating drug sensitivity.

a) Base editing efficiency and precision mapped across 45 endogenous loci in HT-29 CBE and ABE cells. Average VAFs for exact edits for hit gRNAs are shown for each variant from amplicon sequencing data that were absent in unedited samples. Dashed lines represent the predicted base editing activity window. Data represent the mean of two independent experiments performed on separate days. VAF, variant allele frequency. b) Editing efficiency and precision of CBE and ABE base editors are shown by amplicon sequencing of endogenous DNA loci. Base editing was performed by doxycycline-induced expression of ABE (top panel) or CBE (bottom panel) for three days. Rare transversion mutations and their sequence context within the gRNA are highlighted by a red box. VAF, variant allele frequency from amplicon sequencing and represent the mean of two independent experiments.