Fig. 6: Altered cell–cell communication signaling networks in DSAD. | Nature Genetics

Fig. 6: Altered cell–cell communication signaling networks in DSAD.

From: Spatial and single-nucleus transcriptomic analysis of genetic and sporadic forms of Alzheimer’s disease

Fig. 6: Altered cell–cell communication signaling networks in DSAD.

a, Schematic representation of CCC analysis. b, Bar plot showing signaling pathways with significant differences between DSAD and controls, ranked based on their information flow (sum of communication probability among all pairs of cell populations in the network). c,d, Network plot showing the CCC signaling strength between different cell populations in controls (c) and DSAD (d) for NECTIN signaling. e, Spatial feature plots of the snRNA-seq in predicted spatial coordinates for one control sample (left) and one DSAD sample (right) for select NECTIN signaling genes. f, Dot plot showing gene expression of NECTIN signaling genes with significant CCC interactions in control (top) and DSAD (bottom). g, Representative double immunofluorescence images for Nectin2 (green), Map2 (yellow) and DAPI (blue) from postmortem human brain tissue (PFC) of control, late-stage AD and DSAD cases. h, Bar plot representing results of NECTIN colocalization analysis from ×60 images (n = 5 cognitively healthy control, n = 4 late-stage AD and n = 4 DSAD cases) using the JACoP Plugin from ImageJ and Manders' correlation coefficient. Data are presented as the average of three different fields of view (FOVs) per sample. P values from two-way t tests are shown. Error bar shows one s.d. from the mean. i,j, Network plot as in c and d in control (i) and DSAD (j) for ANGPTL signaling. k, Spatial feature plots as in e for one control sample (top) and one DSAD sample (bottom) for the ANGPTL pathway. l, Dot plot showing gene expression of ANGPTL signaling genes with significant CCC interactions in control (top) and DSAD (bottom). m, Representative double immunofluorescence images at ×10 and ×60 magnification for ANGPTL4 (green), GFAP (red) and DAPI (blue) from postmortem human brain tissue (PFC) of control, late-stage AD and DSAD cases. n, Bar plot representing results of ANGPTL colocalization analysis from ×60 images (n = 3 cognitively healthy control, n = 3 late-stage AD and n = 4 DSAD cases) using the JACoP Plugin from ImageJ and Manders' correlation coefficient. Data are presented as the average of three different FOVs per sample. P values from two-way t tests are shown. Error bar shows 1 s.d. from the mean.

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