Extended Data Fig. 3: Cells at different stages of S phase display different replication-induced fluctuations of RDR.
From: Characterizing the evolutionary dynamics of cancer proliferation in single-cell clones with SPRINTER
Average RDRs (y axis) were measured by SPRINTER in 50 kb genomic bins with either early (magenta) or late (green) replication timing across autosomes in the genome (x axis) for (a) 180 early-S-phase cells, (b) 916 mid-S-phase cells and (c) 901 late-S-phase cells in the generated tetraploid ground truth dataset that were identified as S phase by SPRINTER. As expected, cells at different stages of S phase exhibit clearly different replication fluctuations in RDRs: in early-S phase only early-replicating bins shift to higher values of RDR, in mid-S phase all the early bins have completed replication and have distinctly higher values of RDR than late bins, and in late-S phase, late bins also start replicating and some of these bins increase their values of RDR.
