Fig. 5: An active chromatin structure connects convergent promoters.
a–g, Summary profiles (top panels) and heatmaps with individual convergent promoter regions (bottom panels) display epigenetic signals at MCF-7 convergent promoters. Convergent promoters are length-sorted in descending order. a, ATAC–seq signals. Left, original scale with start (TSS1) and end (TSS4) indicated by dashed lines. Right, all regions adjusted to the same scale with start (TSS2) and end (TSS3) marked by dashed lines. b–g, CpG islands (b), H3K4me3 (c), H3K9ac (d), H2AFZ (e), H3K27ac (f) and H3K4me1 (g) signal P values (−log10) at scale-adjusted regions. Negative decadic logarithms of signal P values computed using a Poisson model-based statistical test were obtained directly from ENCODE data files. h, Differential ATAC–seq signal (log2FC Nutlin-3a compared with DMSO control) at downstream promoter compared with its upstream promoter counterpart in MCF-7 cells. Linear regression with 95% confidence intervals. Spearman correlation with two-tailed significance.
