Extended Data Fig. 3: Examples of non-classical adipocytes from additional patients.

A-C. Representative images of adipocytes displaying positive signal for the different markers used to identify the different adipocytes subpopulations (PDE4D (A); SKAP1 (B); ANK2 (C)) taken from additional patients, with an additional zoomed out images to illustrate prevalence and localization. Thin scale bar = 20 μm. Thick scale bar = 200 μm. D. Similar analyses for validating VA6 using the protein marker encoded by the mitochondrial gene MT-CO2, with CD36, instead of PLIN1, for staining adipocytes (*), for technical (antibody compatibility) purposes. Shown are representative images of adipocytes from 6-8 different patients (from at least 2 independent staining experiments).