Fig. 2: Adipocyte-specific atlases of hSAT and hVAT. | Nature Genetics

Fig. 2: Adipocyte-specific atlases of hSAT and hVAT.

From: Human subcutaneous and visceral adipocyte atlases uncover classical and nonclassical adipocytes and depot-specific patterns

Fig. 2

a, UMAP representations of hSAT and hVAT adipocyte atlases containing 12,205 and 15,460 nuclei, respectively. Pie charts show the relative abundance of each adipocyte subset. b, The expression of adipocyte markers in adipocyte clusters of each depot. Distinct clusters expressed markers at similar levels. c, Heatmaps based on the top 20 markers of each adipocyte cluster in hSAT and hVAT. Adipocyte clusters are represented by all their nuclei (columns), except for SA1 and VA1 which are represented by a randomly selected subset of nuclei as a result of their large size. d, Violin plots depicting below-threshold DoubletFinder score (upper) and mitochondrial (MT) content (lower) per adipocyte cluster. Clusters that were validated by immunofluorescence (Fig. 5) were marked with ‘+’. e, UMAP representation of the integrated hSAT and hVAT adipocyte atlases, showing that most subsets overlap between the two depots. f, The proportions of SA1–7 and VA1–8 in each iASV1–7 cluster. The total number of nuclei per integrated cluster appears below the cluster name. g, The number of nuclei from SA1–7 and VA1–8 (columns) that contribute to each integrated cluster (rows). Per column, the entry corresponding to the maximal value (that is, most nuclei) is highlighted.

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