Extended Data Fig. 1: The TCA cycle and OXPHOS are related to anti-tumor immunity and changes in metabolites regulate tumor growth.
From: Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma
a, KEGG and hallmark gene set enrichment by GSEA for protein expression show responders enriched in OXPHOS and the TCA cycle, while non-responders are enriched in splicing and DNA replication pathways (FDR < 0.05). b, Scatter plot of Spearman’s rank correlation reveals associations between the TCA cycle/OXPHOS and total immune infiltration in melanoma proteomic data (n = 154). c, Body weight changes in tumor-bearing mice supplemented with succinate and AKG (n = 5/group). d–i, Tumor growth curves, final tumor weights, and mice body weights in B16F10-OVA (d, e), LLC (f, g), or MC38 (h, i) tumor-bearing mice treated with Ctrl, DES, or DMK (n = 5/group). j, Succinate and AKG levels in plasma (n = 5/group) and different tissues (n = 4/group) from tumor-bearing mice 1 hour after DES or DMK (1000 mg/kg) injections, measured via mass spectrometry. k–m Quantification of CD8⁺/CD3+ and GZMB⁺/CD8⁺ T-cell percentages in B16F10-OVA (k), LLC (l), or MC38 (m) tumor-bearing mice under these treatments (n = 5/group). For details on visualization, statistics and reproducibility, see Methods. Note: n refers to independent biological replicates in all experiments.
