Extended Data Fig. 9: PROX1 loss can enhance HCC liver tumor formation in mice.
a, Overall survival of patients with HCC and high PROX1 expression (40% expression cutoff) compared to CCA. b, Representative mouse livers at endpoint of HCC induction by Myc OE and Trp53 KO using HDTVI with PX330-sgRNA-Prox1 KO compared to control (n ≥ 3). c, Number of tumors upon treatment as in b following Prox1 KO vs control (Ctr; n = 3). d, Survival curve of HCC mice treated as in b comparing Prox1 KO (n = 4) with control (n = 3). e, Mouse livers at endpoint of HCC modeling as in b with EF1a-GFP-shProx1 KD compared to control (n ≥ 3). f, Number of tumor nodules with diameter >0.2 mm treated as in e at day 14 (n = 5). g, Tumor size (cross-sectional area) treated as in e in tumors with or without GFP expression (n = 5). h, Tumor numbers upon treatment as in e (n = 5). i, Survival curve of HCC mice treated as in e (n = 5). Scale bar = 10 mm (b,e). Bar graphs show mean from specified biological replicates, error bars = SD, log-rank test (a,d,i), unpaired two-tailed t test (c,g,h) and Bonferroni correction (g). P values are displayed.
