Fig. 7: PROX1 regulates HCC versus CCA fate trajectories.

a, PROX1 expression in patients with HCC and CCA24. The figure is created with BioRender.com. b, Pearson correlation of indicated markers with PROX1 expression in patients from a. c, Immunohistology of HCC (Myc/Trp53) and CCA (Akt/Notch) mice at the endpoint following Prox1 KD or OE compared to control (n = 5). d, Quantification of KRT19+ and HNF4+ cells in GFP+ tumors from c (n = 5, multiple regions per liver). e, Selected differentially expressed genes following RNA-seq from mice in (c) Prox1 KD (n = 2) or Late Prox1 OE (n = 2–3). Scale bar = 50 µm (c). Bar graphs and scatter plots show mean from specified replicates, error bars = s.d., unpaired two-tailed t test and P values are displayed. HE, hematoxylin and eosin.