Extended Data Fig. 8: Association of selection with infiltrating B cell fraction.

a, Volcano plots for the significance of association of IgM/IgD and IgG infiltrating B cell fraction for selection of nonsynonymous genes from the cancer gene census as measured with Poisson model: observed mutations ~ offset(log(expected mutation)) + age + sex + purity + tumor mutation burden + disease type + infiltrating B cells, with expected mutations calculated by dNdScv, and run on the entire pan-cancer cohort excluding hematological and childhood cancers. Dashed black lines are at estimate = 0, and the FDR significance threshold of −log₁₀(P) = 4.16. b, Bubble plot showing disease-type-specific significance from Poisson model for different infiltrating B cell fractions, with genes selected as those that are significant either at the pan-cancer level (MUC4 in IgM/IgD and KMT2C in IgG) or within a single disease-type at an adjusted P < 0.05 with genes only tested if 10 or more patients had nonsynonymous mutations within that gene in that cancer type. P values in a and b represent the significance of the term for the TCRA T cell fraction variable in the Poisson model and are calculated using a Wald test.